Hidehiro Fukaki, Ph.D.

Professor, Division of Biomolecular Organization, Cell Function

Research Interests

Lateral root (LR) formation is one of the post-embryonic developmental processes in vascular plants, which contributes to the establishment of the root system for efficient water and nutrient uptake from the soil, to support shoot development above the ground. To understand the mechanisms of LR formation in vascular plants, we are studying a variety of mutants affecting LR formationin Arabidopsis thaliana. Our recent studies have revealed that the auxin response mediated by SOLITARY-ROOT (SLR)/IAA14-AUXIN RESPONSE FACTOR7 (ARF7)/ARF19 module regulates LR initiation via activation of LBD16/ASL18 and related LBD/ASL genes. This study will elucidate the molecular cascades of auxin-mediated LR initiation and LR primordium development in vascular plants.
     In addition, we are studying the root radial patterning using A. thaliana and Cardamine speicies. This study will unravel the evolutionary mechanism for organ radial patterning in vascular plants.

Research Focus

- Root growth and development in vascular plants
- Auxin signaling in vascular plants

Recent Publications

  • Goto, C., Ikegami, A., Goh, T., Maruyama, K., Kasahara, H., Takebayashi, Y., Kamiya, Y., Toyokura, K., Kondo, Y., Ishizaki, K., Mimura, T., and Fukaki, H. (2023)
    Genetic interaction between Arabidopsis SUR2/CYP83B1 and GNOM indicates the importance of stabilizing local auxin accumulation in lateral root initiation.
    Plant Cell Physiol. , 2023 Jul 31, doi: 10.1093/pcp/pcad084.
  • Goh, T., Toyokura, K., Yamaguchi, N., Okamoto, Y., Uehara, T., Kaneko, S., Takebayashi, Y., Kasahara, H., Ikeyama, Y., Okushima, Y., Nakajima, K., Mimura, T., Tasaka, M. and Fukaki, H. (2019)
    Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana.
    New Phytologist, 224, 749-760.
  • Toyokura, K., Goh, T., Shinohara, H., Shinoda, A., Kondo, Y., Okamoto, Y., Uehara, T., Fujimoto, K., Okushima, Y., Ikeyama, Y., Nakajima, K., Mimura, T., Tasaka, M., Matsubayashi, Y. and Fukaki, H. (2019)
    Lateral inhibition by a peptide hormone-receptor cascade during Arabidopsis lateral root founder cell formation.
    Developmental Cell, 48, 64-75.
  • Orosa-Puente, B., Leftley, N., von Wangenheim, D., Banda, J., Srivastava, A,K., Hill, K., Truskina, J., Bhosale, R., Morris, E.,Srivastava, M., Kümpers, B., Goh, T., Fukaki, H., Vermeer, J.E.M., Teva Vernoux, Dinneny, J.R., French, A.P., Bishopp, A., Sadanandom, A., and Bennett, M.J. (2018)
    Root branching toward water involves posttranslational modification of transcription factor ARF7.
    Science, 362, 1407-1410.
  • Goh, T., Toyokura, K., Wells, D.M., Swarup, K., Yamamoto, M., Mimura, T., Weijers, D., Fukaki, H., Laplaze, L., Bennett, M.J., and Guyomarc'h, S. (2016)
    Quiescent center establishment in Arabidopsis lateral root coincides with developmental phase transition to promote organ emergence.
    Development, 143, 3363-3371.
  • Ito, J., Fukaki, H., Onoda, M. Li, C., Li, L., Tasakam M. and Furutani, M. (2016)
    Auxin-dependent compositional change in Mediator in ARF7 and ARF19 mediated transcription.
    Proc. Natl. Acad. Sci. U.S.A, 113, 6562-6567.
  • Chen, Q., Liu, Y., Maere, S., Lee, E., Van Isterdael, G., Xie, Z., Xuan, W., Lucas, J., Vassileva, V., Kitakura, S., Marhavý, P., Wabnik, K., Geldner, N., Benková, E., Le, J., Fukaki, H., Grotewold, E., Li, C., Friml, J., Sack, F., Beeckman, T., and Vanneste, S. (2015)
    A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development.
    Nature Communications, 6, 8821.
  • Voß, U., Wilson, M.H., Kenobi, K., Gould, P.D., Robertson, F.C., Peer, W.A., Lucas, M., Swarup, K., Casimiro, I., Holman, T.J., Wells, D.M., Péret, B., Goh, T., Fukaki, H., Hodgman, T.C., Laplaze, L., Halliday, K.J., Ljung, K., Murphy, A.G., Hall, A.J., Webb, A.A.R. and Bennett, M.J. (2015)
    The circadian clock rephases during lateral root organ initiation in Arabidopsis thaliana.
    Nature Communications, 6, 7641.
  • Lavenus, J., Goh, T., Guyomarc'h, S., Hill, K., Lucas, M., Voss, U., Kenobi, K., Wilson, M., Farcot, E., Hagen, G., Guilfoyle, T.J., Fukaki, H., Laplaze, L. and Bennett, M.J. (2015)
    Inference of the Arabidopsis lateral root gene regulatory network reveals a bifurcation mechanism that defines primordia flanking and central zones.
    Plant Cell , 27, 1368-1388.
  • Okumura, K.-i., Goh, T., Toyokura, K., Kasahara, H., Takebayashi, Y., Mimura, T., Kamiya, Y. and Fukaki, H. (2013)
    GNOM/FEWER ROOTS is required for the establishment of an auxin response maximum for Arabidopsis lateral root initiation.
    Plant Cell Physiol., 54, 406-417
  • Goh, T., Kasahara H., Mimura, T., Kamiya Y. and Fukaki, H. (2012)
    Multiple Aux/IAA-ARF modules regulate lateral root formation: the role of Arabidopsis SHY2/IAA3-mediated auxin signaling.
    Phil. Trans. R. Soc. Lond. B Biol. Sci. 367, 1461-1468.


Office: Bld C, room 501

Tel: +81-78-803-5721

Fax: +81-78-803-5721

E-mail: h-fukaki@port.kobe-u.ac.jp

URL: http://www.research.kobe-u.ac.jp/fsci-fukaki/fukaki/fukaki_laboratory.html